Intestinal organoids are a key innovative technology to replace animal testing and measure complex traits in animal breeding and nutrition. The objective of this project is to realize a more robust and versatile in vitro system that will allow us to measure complex traits and test the bio-functionality of various dietary components, including nutrients. One of the advantages of such a model is that it retains the genetic information of the individual pig, which can help researchers plan in vitro studies using organoids from different pigs that represent the variation present in the pig population. We anticipate that the use of such a near-physiological in vitro system will result in fewer animals needed for experiments and will contribute to animal breeding and nutrition research. Participating partners of Breed4Food are Topigs Norsvin and Wageningen University & Research.