Current protocols for cryopreservation (vitrification) of gonadal tissue and embryos are suboptimal and there is significant scope for improvement. In the coming years, livestock breeding companies will intensify their breeding efforts towards a climate neutral and circular future agriculture. To achieve this goal, it is very important to further develop and implement innovative Reproductive Technologies (RT). At the same time, improved RT are needed for preserving genetic diversity, selection lines and (rare) breeds. Both breeding companies and the national gene bank (CGN) will greatly benefit from advances in RT. Participating partners of Breed4Food are CRV, Topigs Norsvin and Wageningen University & Research.